Chromatography

ChemistryAQAGCSEUnit: Chemical Analysis
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The basics

The Science Behind Solving Crimes

🕵️ The Science Behind Solving Crimes

In 1995, a forger was caught because forensic scientists ran chromatography on ink from a document. The ink produced three spots — the forger had mixed different inks to try to match the original. The genuine signature produced only one spot. Paper chromatography, a technique invented in 1944, uses nothing more than paper and solvent to separate mixtures into their individual components. Today it helps catch criminals, test food dyes, and quality-check medicines.

What is chromatography?: A separation technique used to separate mixtures into their individual components based on different affinities for mobile and stationary phases
Key terms

Chemistry glossary

What is chromatography?
A separation technique used to separate mixtures into their individual components based on different affinities for mobile and stationary phases
What does Rf stand for and what does it measure?
Rf = Retention factor. It measures how far a substance travels compared to the solvent (distance moved by substance ÷ distance moved by solvent)
Spotlight
What is Chromatography?

Chromatography is a separation technique used to separate mixtures into their individual components and to identify those components.

Exam tip

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🧠 Memory Aid

Rf formula: "Rf = substance over Front"

Rf = distance by Substance ÷ distance by solvent Front
The capital S on top, capital F on bottom — "Substance over Front"

For what moves where: "More Soluble = Sprints further up"

For phases: "Mobile MOVES, Stationary STAYS"

Quick Check: A student runs chromatography on a suspected food dye and gets three spots. What does this tell them?

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Quiz · Question 1 of 23

What is the purpose of chromatography?

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