Required Practical: Measuring Decomposition Rate

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๐Ÿงช Required Practical: Measuring Decomposition Rate

Painted lab apparatus for the decomposition required practical: two conical flasks side by side, each with a cotton wool plug and containing cloudy milk with lipase enzyme and a pH indicator. LEFT flask sits in a warm water bath at 35ยฐC with steam rising. RIGHT flask sits in an ice bath at 5ยฐC with snowflakes drifting. Painted parchment lists the experimental design โ€” independent variable: temperature; dependent variable: time for milk to clear; control variables: pH, milk volume, enzyme concentration. Decomposition is faster at warmer temperatures up to around 40ยฐC.

This practical investigates how temperature affects decomposition by measuring the time taken for lipase enzyme to break down the lipids in milk. A pH indicator (e.g., cresol red) changes colour as fatty acids are released, allowing the rate of decomposition to be timed at different temperatures.

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