Cell Organelles

Scientists studying mitochondria use electron microscopes rather than light microscopes. Which statement correctly explains why electron microscopes are more useful for studying cell ultrastructure?

• A. Electron microscopes produce a coloured image that makes organelles easier to identify
• B. Electron microscopes have a higher resolution, so finer details of organelles can be seen
• C. Electron microscopes allow scientists to study living cells in real time
• D. Electron microscopes are cheaper and easier to use than light microscopes

A researcher is isolating mitochondria from liver cells using cell fractionation. The protocol states the homogenisation solution must be: (i) cold, (ii) isotonic, and (iii) buffered. Explain why each of these three conditions is necessary.