Cell BiologyVery High Exam FrequencyAQAEdexcelOCRWJEC

Microscopy

Light and electron microscopes, magnification and resolution calculations, specimen preparation, staining techniques, and practical microscopy skills

What you'll cover

  • Magnification definition and formula
  • Resolution definition and limiting factors
  • Light microscope parts and function
  • Correct microscope technique and method
  • Unit conversions (mm, μm, nm)
  • Magnification calculations (total, actual size, image size)
  • Specimen preparation and staining
  • Coverslip technique to avoid air bubbles
  • TEM vs SEM comparison
  • Electron microscope advantages and limitations
  • Cell size ranges and typical measurements
  • Microscopy troubleshooting and common problems

Sample Flashcards

What is resolution?
Resolution is the ability to distinguish between two separate points that are close together. It determines how clear and detailed an image is.
What is magnification?
Magnification is how many times larger an image appears compared to the actual object. It tells us how much bigger something looks through a microscope.

Sample Questions

What is magnification?

  • A. The ability to distinguish between two separate points
  • B. How many times larger an image appears compared to the actual object
  • C. The brightness of an image under a microscope
  • D. The wavelength of light used in microscopy
1 markfoundation

Explain why specimens are stained before viewing under a light microscope.

3 marksstandard

18

exam-style questions

20

revision flashcards

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