Cell BiologyRequired Practical

Required Practical: Food Tests (Benedict's, Biuret, Iodine)

Part of Cell Biology Practical Investigations · GCSE GCSE Biology revision

This required practical covers Required Practical: Food Tests (Benedict's, Biuret, Iodine) within Cell Biology Practical Investigations for GCSE Biology. Comprehensive practical skills, experimental design, data analysis, microscopy techniques, and scientific methodology in cell biology It is section 6 of 18 in this topic. Revise both the method and the reason for each step, because practical questions often test understanding rather than pure recall.

Topic position

Section 6 of 18

Practice

20 questions

Recall

20 flashcards

Required Practical: Food Tests (Benedict's, Biuret, Iodine)

Painted required practical apparatus showing the three food tests: three pairs of test tubes on a wooden rack, each pair showing the before-and-after colour change. Benedict's test for reducing sugars (blue heated in water bath turns brick red). Biuret test for proteins (pale blue turns purple, no heating). Iodine test for starch (yellow-brown turns dark blue-black, no heating). Parchment notes that Benedict's requires water-bath heating while biuret and iodine do not, and to always test for all three when asked to identify an unknown food.

What each test detects

  • Benedict's reagent — tests for reducing sugars (e.g. glucose). Heat the sample with Benedict's in a water bath. Positive result: blue → brick red. Negative: stays blue.
  • Biuret reagent — tests for proteins. Add biuret to sample, no heating. Positive result: pale blue → purple/lilac. Negative: stays blue.
  • Iodine solution — tests for starch. Add iodine to sample, no heating. Positive result: yellow-brown → blue-black. Negative: stays yellow-brown.

Exam tip

When asked to identify an unknown food, perform all three tests on separate samples. Examiners often give a food that contains MORE than one nutrient — students who only do one test miss marks. Also note the heating step: only Benedict's requires a water bath. Forgetting to heat Benedict's (or wrongly heating biuret/iodine) is a common mark-losing error.

Keep building this topic

Read this section alongside the surrounding pages in Cell Biology Practical Investigations. That gives you the full topic sequence instead of a single isolated revision point.

Practice Questions for Cell Biology Practical Investigations

When using a light microscope to observe cells, which objective lens should be used first?

  • A. The lowest power objective lens
  • B. The highest power objective lens
  • C. The medium power objective lens
  • D. Any objective lens can be used first
1 markfoundation

Describe how to focus a light microscope on a specimen.

3 marksstandard

Quick Recall Flashcards

What is the formula for percentage change in mass?
Percentage change = ((Final mass - Initial mass) ÷ Initial mass) × 100
What is the formula for magnification?
Magnification = Image Size ÷ Actual Size

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